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After elimination of the Transwell insert from the plate, 70% ethanol was extra for 10 min for cell fixation and crystal violet was extra to stain the invading colony. Briefly, cells ended up plated on leading of the filter membrane in a Transwell insert and incubated at 37°C and 5% CO2 for ten min to make it possible for them to settle. After centrifugation, the cells ended up resuspended and incubated with PI/RNase staining buffer for 15 min. After centrifugation, the cells ended up washed with PBS followed by PI/RNAse staining buffer (BD Biosciences, San Jose, CA). Cells ( cells/effectively) ended up plated on to 24-well dishes in triplicate and incubated overnight. Briefly, four types of human colon most cancers cells have been seeded onto a 96-nicely society plate at cells/effectively and incubated for forty eight h to allow entire attachment. In quick, the cells have been addressed with , 1.25, and 2.5 mg/ml of sorghum extract for forty eight h in total medium made up of 10% FBS. After washing to take out debris, the cells were being incubated with refreshing complete medium containing , 1.25, and 2.5 mg/ml high phenolic sorghum bran extract.